Penicillin

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Alexander Fleming accidentally created bacteria-free penicillin annulus in staphylococcus culture even if diluted eight-hundredfold, 1928

Large-scale production of penicillin is achieved using submerged fermentation; mold is grown in agitated and aerated tanks, rather than on the medium surface. (Abbott; Lederle; Merck; Pfizer; Squibb), 1943